Method for the diagnosis of papillary renal cell carcinoma and a kit for its implementation

摘要

1. A method for diagnosing papillary renal cell carcinoma (PPC), comprising the following steps: a) obtaining an initial pair of tissue samples from a patient, where one of the samples was obtained from tissue suspected of being affected by cancer, and the second was obtained from adjacent histologically normal tissue (“conditional norm”) ; b) isolation and purification of RNA preparations from the initial pair of samples; c) synthesis of single-stranded or double-stranded cDNA on an RNA matrix using oligonucleotide primers; d) quantitative amplification of the on CALL using the cDNA obtained in step c) as a template and a pair of gene-specific oligonucleotide primers SEQ ID NO: 1 and 2; e) comparing the amount of amplified CALL DNA fragment for a sample obtained from tissue suspected of being affected by cancer with the amount of amplified a DNA fragment for a sample obtained from normal tissue, where the indicated amounts of the amplified DNA fragment reflect a change in the CALL gene mRNA content, and a decrease in the CALL gene mRNA is a diagnostic sign of AUC. . The method of claim 1, wherein the sequence of primers and probe is represented by SEQ ID NO: 1, 2, and 3.3. The method of claim 1, wherein, in step d), the quantitative amplification reaction of the CALL gene fragment is real-time PCR. The method of claim 1, wherein in step e), GUSB and / or RPN1 genes encoding beta-glucuronidase and riboforin 1, respectively, are used as internal control for kidney tissue. A set of primers and probe for real-time polymerase chain reaction for the quantitative assessment of the mRNA content of the CALL gene having the sequence of SEQ ID NO: 1, 2 and 3.