A method for determining SH-groups in the membranes of erythrocytes of the peripheral blood, which allows to determine the degree of stability of erythrocytes and hemoglobin oxygenation

摘要

A method for determining SH-groups in membranes of erythrocytes of the peripheral blood, comprising executing histochemical reaction in the incubation solution consisting of a mixture of: 1-25 mg dihydroxy-binaphthyl-disulfide in 15 ml of absolute alcohol and 2-35 ml of a 0.1 M veronal acetate buffer in which a screen 500 making .mu.l 200 .mu.l whole blood coagulant and incubated at 56 ° C for 1 hour, then allowed to stand at room temperature for 10 minutes. and the centrifuged precipitate was washed in distilled water, and then twice in 0.01% acetic acid and twice with 96 ° C in an alcohol containing 5 minutes in ether, washed in 96 ° ethanol and then in distilled water and the precipitate was placed for 10 minutes in lasting blue solution of 0.1 M phosphate buffer pH 7,6-7,8 rate of 1 mg / ml; rinsed for 2 minutes. in running water and then in distilled, drained and the excess supernatant is removed and from the remaining slurry prepared monolayer smears on which study computer cytophotometry method contents SH-groups in the membranes of erythrocytes and it is established that during exacerbation CMV infection to a titer of 1: 1600 the number of SH-groups in the membranes of erythrocytes is reduced to 10.50 ± 0.20 usl. u, which increases in the peripheral blood to 12.00 ± 0.09% degenerative forms of erythrocytes and the content HbOsnizhaetsya to 91.2 ± 1.3%.