METHOD FOR EXPRESS ANALYSIS OF GENETIC POLYMORPHISM FOR IDENTIFICATION OF A GENETIC POSITION FOR A DIAGNOSED DISEASE AND BIOCHIP

摘要

1. A method for the rapid analysis of genetic polymorphism to identify a genetic predisposition to the diagnosed disease, including verification of pathogenic mutations in a clinical nucleic acid (NK) sample by differentiating hybridization of labeled probes from a patient's NK, comprising the following stages: immobilization of oligonucleotides on a microchip; isolation of nucleic acids from a patient’s blood sample; obtaining a labeled probe for hybridization; hybridization of the obtained labeled probe with immobilized oligonucleotide crochyp; washing of a non-specifically bound probe under conditions discriminating mutant genotypes; scanning the microchip and obtaining results; general assessment of the analysis results; characterized in that oligonucleotides 25-60 bases in length containing and not containing a genetic marker are immobilized (3 repetitions each oligonucleotide), as well as oligonucleotides from genes of the human household and from genes of other organisms that do not have homology in the human genome; the labeled probe is obtained by reverse transcription reactions with simultaneous labeling with a fluorescent label for RNA as a clinical sample, or nick-translation labeling for DNA as a clinical sample, using total RNA or DNA. 2. The method according to p. 1, characterized in that as the oligonucleotides for immobilization on a microchip containing and not containing a genetic marker, oligonucleotides with the sequence shown in table are used. 1.3. The method according to p. 1, characterized in that as oligonucleotides for immobilization on a microchip, soda