A method of obtaining a biologically active concentrate from raw deer antlers (options)

摘要

1. A method of obtaining a biologically active concentrate from raw deer antlers, characterized in that the raw antlers are crushed and carried out sequentially, in three stages, water extraction during breeding of raw materials: water 1: 1-1: 2 under the influence of ultrasonic vibrations with a frequency of from 20 to 50 kHz, followed by filtration and vacuum drying of the hydrolysates, while in the first stage, water extraction is carried out at a temperature not exceeding 39 ° C for 1-3 hours, in the second stage, the extraction is carried out in the process of enzymatic hydrolysis of oilcake After the first stage in the presence of the pepsin enzyme, in the third stage, the extraction of cake is carried out after the second stage of extraction in the presence of the papain enzyme, the duration of the process at each stage is 3-5 hours and the concentration of enzymes in the substrate is 0.8-1.2% .2. The method according to claim 1, characterized in that the raw antlers are cleaned of mechanical impurities, washed and dried at room temperature, ground to a particle size of 5-20 mm. The method according to claim 1, characterized in that the enzymatic hydrolysis in the presence of the pepsin enzyme is carried out at a pH of 1-3 at a temperature of 32-39 ° C, in the presence of a papain enzyme with an activity of 30 thousand units at a pH of 5-7.5 at a temperature of 30 -40 ° C. 4. The method according to claim 1, characterized in that the vacuum drying of the hydrolysates after three stages of extraction is carried out at a temperature of not higher than 40 ° C and a pressure of 0.9 atm., And the vacuum drying of the hydrolyzate after high-temperature extraction at a temperature of not higher than 50 ° C and a pressure of 0 9 atm. 5. A method of obtaining a biologically active concentrate from raw deer antlers, characterized in that the raw antlers are crushed and carried out sequentially, in three stages, water extraction during breeding of raw materials: water 1: 1-1: 2 under the air