RECOMBINED PLASMID DNA CODING CHIMERIC ANTIBODY AGAINST HUMAN TUMOUR NECROSIS FACTOR-ALPHA, EUKARYOTIC CELL LINE PRODUCING CHIMERIC ANTIBODY, AND METHOD OF CHIMERIC ANTIBODY OBTAINMENT

摘要

FIELD: biotechnologies.SUBSTANCE: invention offers recombinant plasmid DNA coding a chimeric antibody against human tumour necrosis factor-alpha (TNF-alpha) based on pOptiVEC-TOPO® plasmid. Invention refers to eukaryotic cell line as a producer of antibody to TNF-alpha, method of cell line obtainment by transfection of plasmid DNA according to the invention, and method of chimeric antibody obtainment for TNF-alpha by cultivation of cell line according to the invention.EFFECT: increased synthesis level for antibodies against TNF-alpha by producer cells.12 cl, 8 dwg, 1 tbl, 3 ex