RECOMBINANT DNA pA4, RECOMBINANT DNA pQE 30-pA4, PROVIDING OBTAINING POLYPEPTIDE A4, Esherichia coli STRAIN M 15-A4, TRANSFORMED WITH RECOMBINANT PLASMID DNA pQE 30-pA4, AND EXPRESSING RECOMBINANT POLYPEPTIDE A4, RECOMBINANT POLYPEPTIDE A4, POSSESSING ABILITY TO SELECTIVELY BIND HSA, AFFINE SORBENTS (VERSIONS) AND METHODS FOR HSA AND IgG REMOVAL FROM BLOOD SERUM (VERSIONS)

摘要

FIELD: chemistry.;SUBSTANCE: inventions relate to field of molecular biology and deal with recombinant DNA pA4, recombinant plasmid DNA pQE 30-A4, Esherichia coli strain M15-A4, recombinant polypeptide A4, possessing ability to selectively bind human serum albumin (HSA), affine sorbent, containing such polypeptide, affine combined sorbent and methods for successive removal of HSA and IgG from blood serum. Characterised affine combines sorbent is obtained by mixing affine sorbent, containing said recombinant polypeptide A4, and analogous affine sorbent, in which known IgG-binding polypeptide is used as ligand.;EFFECT: claimed inventions can be used in medical practice to deplete blood serum of two proteins, albumin (HSA) and immunoglobulin G (IgG), present in it in high concentrations Removal of two major proteins from blood serum will make it possible to identify other proteins, present in serum in lower concentrations.;8 cl, 9 dwg, 7 ex, 1 tbl