Recombinant DNA pG4223 and pG14223, recombinant plasmid DNA pQE 30-pG4223 and pQE 30-pG14223, providing for the G4223 and G14223 polypeptides, strains of Esherichia coli M 15-G4223 and Esherichia coli M 15-G14223, transfaccinated strains M 15-G4223 and Esherichia coli M 15-G14223; pQE 30-pG14223, expressing recombinant G4223 and G14223 polypeptides, recombinant G4223 and G14223 polypeptides, with the ability to selectively bind IgG and their use as ligands in affinity chromatography for isolating IgG from blood serum

摘要

FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology and to obtaining a genetic construct which provides synthesis of Escherichia coli recombinant polypeptide G4223 (pG4223). Disclosed are recombinant DNA pG4223, having nucleotide sequence SEQ ID NO: 1 and coding pG4223, characterized by amino acid sequence SEQ ID NO: 2 and capable of selectively binding human immunoglobulin G (IgG); recombinant plasmid DNA pQE 30-pG4223, which is plasmid pQE 30 carrying a recombinant DNA pG4223, which enables to obtain pG4223; strain E. coli M15-G4223, producing pG4223, obtained on the basis of E. coli strain M 15 and containing recombinant plasmid pQE30-pG4223; polypeptide pG4223 with amino acid sequence SEQ ID NO: 2, capable of selectively binding IgG, and use thereof as a ligand in an affinity sorbent for removal of IgG from blood serum and simultaneously for producing a purified IgG preparation.EFFECT: invention enables to obtain an IgG-binding recombinant polypeptide G4223 capable of selectively binding the Fc portion of IgG of most species of mammals.6 cl, 1 tbl, 4 dwg, 6 ex