DIAGNOSTIC TECHNIQUE FOR SEVERITY OF BRONCHIAL ASTHMA AT DECREASE IN SMOOTH MUSCULAR BRONCHIAL MUCOSA MYOGLOBINE PEROXYDASE ACTIVITY

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine ;and concerns diagnostic technique for severity of bronchial asthma. Method is implemented by detecting myoglobin peroxidase activity by histochemical method after bronchial mucosa biopsy materials fixing in 10 % formalin solution during 5 days, their freezing and preparation of sections with thickness of 5 mqm, that are placed on 1 min in benzene and stained for 5-8 minutes at 80 °C in solution, consisting of 100 mg benzidine in 0.85 % NaCl, solution is cooled down and filtered, adding to 9 ml of solution 1 ml of 40 % ammonium chloride solution and 1 drop of 3 % hydrogen peroxide solution, after conducted reaction sections are rinsed in 0.85 % NaCl and enclosed in gelatin-glycerin for further study of myoglobin peroxidase activity by "Scion" program.;EFFECT: at reduction of Myoglobin peroxidase activity in smooth muscular bronchi to 27,5 ±1,1 standard units, reduction of bronchial lumen to 55,0±2,3%, reduction of LVC to 69,1 ±4,2 %, FVC to 64,2±3,8 %, FEV₁ to 45,7±4,4%, bronchial passibility MVH₇₅ to 32,4 ±2,9% severe form of bronchial asthma is diagnosed.;1 cl, 3 dwg