isolated population of cells, in particular adult stem cells, genetically modified

摘要

The present invention concerns an isolated population of cells characterized by the fact of (I) comprises at least one exogenous RNA (II), to obtain the gene silenced PHD-2, and / or (Illa) have the UQCRB gene silenced or (IIIb) to obtain the SIRT3 gene overexpressed or (IIIC) have one or more of the following systems modulated by the use of inhibitors or inducers: SOD1 / SOD2 / SOD3 (superoxide dismutase 1-3), Nrf2 / keep1 (NF-E2-related transcription factor 2), CAT (catalase), Grx (glutathione-glutaredoxin system), PGC-1α (peroxisome proliferator-activated receptor gamma coactivator 1 alpha) FoxOS (forkhead box protein family), Trx (thioredoxin system), NOX-DUOXs (NADPH oxidase family), phox family PRXs (peroxiredoxins), MPO (myeloperoxidase), glutathione peroxidase (GPx); optionally (IV) comprises at least a suicide gene, and / or (V) having the HIF-1 alpha gene silenced. This isolated population of cells is useful in the treatment of tissue damage, degenerative diseases, inflammatory and immune disorders, bacterial infections, in gene therapy, in the treatment of viral diseases and tumors. A method for producing said isolated population of cells is a further object of the invention.