Mass spectrometric sepsis diagnosis without blood culture

摘要

A process for the identification of microbes in body fluid comprising the steps of: a) dissolving the human particles in the body fluid by a surfactant or destroying the human particles with distilled water such that the microbes remain viable in the body fluid; b) separating the microbes from the fluid; c) propagating the microbes in a nutrient broth that does not contain the antimicrobial constituents of the body fluid, d) separating the microbes from the nutrient broth, e) identifying the microbes by similarity comparison of a mass spectrum of the microbial proteins with reference spectra.