A method for the detection of at least of a target analyte in a sample

摘要

The present invention relates to a method for the detection of at least of a target analyte in a sample, andA) a to a solid surface of the immobilized complex is formed, comprisinga) a catcher - adhesion molecule, comprising the target analyte is specifically binds;b) the target analyte is to be detected;c) a reporter - adhesion molecule, wherein the reporter - adhesion molecule (aa) a molecule and (bb) are bonded to it a double-stranded reporter dna, wherein the reporter dna in at least one of their strands i) a first sequence with a recognition sequence for a nicking - endonuclease, and ii) 3 ''thereof has a second sequence, which, as for the reporter - adhesion molecule specific sequence - day serving;B) of the complex formed is washed; andC) a nicking - endonuclease and a polymerase, which is a strand - activity - displacement, however, does not have a 5 ''to 3 exonuclease - activity is added and isothermal amplification of the sequence - tags is effected by the repeated nicking - endonuclease its recognition sequence can be recognized and a pitch in the first sequence of the reporter dna, so that a free 3'' oh end is produced, the 5 ''of the sequence - tags, and of the aircraft pitch by the polymerase with to the counter strand complementary nucleotides with simultaneous displacement of the sequence - tags is filled from the double strand;D) of the displaced sequence - day is detected, as a result of which the detection of the target analyte is carried out in the sample.Furthermore, kit and systems for carrying out the process is made available.