Method and apparatus for reducing the concentration of circulating tumor cells from a cell suspension

摘要

The present invention relates to a process for reducing the concentration of circulating tumor cells from a cell suspension comprising the steps of: providing the cell suspension containing a mixture of cells (11) and depleted tumor cells; incubation of the cell suspension for a time of incubation with biocompatible coated magnetic nanoparticles (10), and after the conclusion of the incubation time a magnetic separation of with magnetic nanoparticles (10) loaded cells (11) as well as of free magnetic nanoparticles (10) from the incubated cell suspension. The method is characterized in that the cell suspension to an incubation temperature tempered and during the incubation over the time of incubation is held on the incubation temperature and the incubation without the addition of a stopping solution is carried out at the end of the incubation time. The present invention also relates to an apparatus for separating off with magnetic nanoparticles (10) loaded cells (11) as well as free of magnetic nanoparticles (10) consists of a cell suspension incubated.