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OPTICAL ANALYSIS DEVICE AND METHOD FOR MICROFLUIDIC DNA ELECTROPHORESIS DETECTION

机译:微流体DNA电泳检测的光学分析装置和方法

摘要

Aspects of the disclosure provide a microfluidic chip. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, and a second domain for electrophoretic separation. The first domain includes at least a first reaction reservoir designated for PCR amplification based on a first sample, and a second reaction reservoir designated for PCR amplification based on a second sample. The second domain includes at least a first separation unit coupled to the first reaction reservoir to received first amplified DNA fragments based on the first sample, and a second separation unit coupled to the second reaction reservoir to received second amplified DNA fragments based on the second sample. The first separation unit is configured to perform electrophoretic separation for the first amplified DNA fragments, and the second separation unit is configured to perform electrophoretic separation for the second amplified DNA fragments.
机译:本公开的方面提供了微流体芯片。该微流体芯片包括被配置用于DNA片段的聚合酶链反应(PCR)扩增的第一结构域,以及用于电泳分离的第二结构域。第一域包括至少一个基于第一样品被指定用于PCR扩增的第一反应容器,和一个基于第二样品被指定用于PCR扩增的第二反应容器。所述第二结构域包括至少一个第一分离单元,其与所述第一反应储库耦合以基于所述第一样品接收第一扩增DNA片段;以及第二分离单元,其与所述第二反应储库耦合以基于所述第二样品接收第二扩增DNA片段。 。第一分离单元被配置为对第一扩增的DNA片段进行电泳分离,第二分离单元被配置为对第二扩增的DNA片段进行电泳分离。

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