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IMMUNOASSAY-BASED DETERMINATION OF IN-SOLUTION BINDING KINETICS
IMMUNOASSAY-BASED DETERMINATION OF IN-SOLUTION BINDING KINETICS
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机译:基于免疫分析的溶液内结合动力学测定
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摘要
Herein is reported a method for the determination of the binding affinity of a binder and its ligand comprising the step of determining based on the result of an immunoassay the fraction of free binder in a sample comprising binder, ligand and binder-ligand-complexes for at least two different binder:ligand ratios in the sample, and if the determined fraction of free binder is not comparable for all used binder:ligand ratios then the binder:ligand ratio in the sample is lowered and the sample is re-analyzed by the same immunoassay, and calculating based on the fraction of free binder in the previous step the binding affinity for the binder to its ligand.
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