COMPOSITIONS OF ENDODERM CELLS AND HEPATIC PARENCHYMAL CELLS AND METHODS FOR OBTAINING AND USING THESE CELLS

摘要

PROBLEM TO BE SOLVED: To provide efficient methods for generating a population of endoderm cells and/or differentiated cells derived from endoderm cells such as hepatic cells, pancreatic progenitor cells, pancreatic cells, intestinal progenitor cells, intestinal cells, lung progenitor cells and lung cells.;SOLUTION: Provided is an isolated population of endoderm cells at least 83% of which are expressing SOX17, at least 77% of which are expressing FoxA2 or at least 76% of which are expressing CXCR4. Also provided is a method for culturing the endoderm cells in a culture medium containing an effective amount of a selective inhibitor of P13Kα and an effective amount of Activin A. The selective inhibitor of P13Kα may be a fused pyrimidine compound of Formula (I), specifically preferable one may be 4-[2-(1H-indazole-4-yl)-6-[(4-methylsulfonyl piperazine-1-yl)methyl]thieno[3,2-d)pyrimidine-4-yl]morpholine.;SELECTED DRAWING: None;COPYRIGHT: (C)2017,JPO&INPIT