Method for identifying interferon having direct inhibitory effect on tumor and use thereof

摘要

An invention related to a predetermined interferon is provided. The present invention provides a novel method for determining or comparing the relative potency of test interferon relative to rSIFN-co (interferon having therapeutic effects on solid tumors); a substantial difference between test interferon and rSIFN-co. Methods for constructing equivalence; Methods for down-regulating expression of Wnt-related receptors or co-receptors such as LRP6 / FZD6; Methods for down-regulating expression of Wnt-related target genes such as Axin2, CD24, survivin and / or ID2 A method of suppressing the transcriptional activity of β-catenin / TCF; a method of suppressing the expression of β-catenin; a tumor suppressor gene such as DKK-3, BATF2 and / or KLF4 is up-regulated to suppress tumor cell viability in vitro Tumor growth and metastasis A method for suppressing tumor cell metastasis, pseudopod formation and colony formation in vitro; a method for determining the efficacy of test interferon, a kit for determining these methods, and an interferon or an interferon substitute having the activity provide. [Selection figure] None