Structural investigation of protein dispersed in liquid phase by Raman spectroscopy

摘要

Method for investigating Raman spectroscopic structure of a sample containing chemical species dispersed in liquid phase, particularly protein, and apparatus for carrying out said method. The method comprises the steps of providing a sample, providing marker particles in the sample, exciting the sample with a light source, receiving Raman scattered light from the dispersed species in the sample, receiving Detecting Raman scattering from the dispersed chemical species in the sample from the Raman scattered light, detecting the movement of the marker particles in the sample, detecting the Raman scattering and detecting the movement of the particle And extracting at least one property of the dispersed chemical species in the sample from both.