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Method for obtaining a cell culture comprising hepatocytes from a group of cells containing hepatocytes differentiated from induced pluripotent stem cells
Method for obtaining a cell culture comprising hepatocytes from a group of cells containing hepatocytes differentiated from induced pluripotent stem cells
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机译:从含有从诱导性多能干细胞分化而来的肝细胞的细胞群中获得包含肝细胞的细胞培养物的方法
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摘要
PROBLEM TO BE SOLVED: To provide a method for obtaining a cell culture substantially consisting of hepatocytes from a cell group which comprises hepatocytes having been differentiation-induced from induced pluripotent stem cells (hereinafter referred to as iPS cells) and undifferentiated iPS cells.SOLUTION: The method comprising a culturing step of a cell group which includes hepatocytes having been differentiation-induced from iPS cells and undifferentiated iPS cells, in a culture medium whose composition includes a serum or a serum substitute: comprises obtaining and sorting a cell culture substantially consisting of hepatocytes from the cell group.
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