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Method for obtaining a cell culture comprising hepatocytes from a group of cells containing hepatocytes differentiated from induced pluripotent stem cells

机译:从含有从诱导性多能干细胞分化而来的肝细胞的细胞群中获得包含肝细胞的细胞培养物的方法

摘要

PROBLEM TO BE SOLVED: To provide a method for obtaining a cell culture substantially consisting of hepatocytes from a cell group which comprises hepatocytes having been differentiation-induced from induced pluripotent stem cells (hereinafter referred to as iPS cells) and undifferentiated iPS cells.SOLUTION: The method comprising a culturing step of a cell group which includes hepatocytes having been differentiation-induced from iPS cells and undifferentiated iPS cells, in a culture medium whose composition includes a serum or a serum substitute: comprises obtaining and sorting a cell culture substantially consisting of hepatocytes from the cell group.
机译:解决的问题:提供一种从基本上由肝细胞组成的细胞组中获得细胞培养物的方法,该细胞组包括从诱导多能干细胞(以下称为iPS细胞)和未分化的iPS细胞中分化诱导而来的肝细胞。该方法包括在组成包括血清或血清替代物的培养基中培养包括从iPS细胞和未分化的iPS细胞分化诱导的肝细胞的细胞群的培养步骤:包括获得和分选基本上由以下组成的细胞培养物:细胞组中的肝细胞。

著录项

  • 公开/公告号JP6057418B2

    专利类型

  • 公开/公告日2017-01-11

    原文格式PDF

  • 申请/专利权人 国立大学法人 千葉大学;

    申请/专利号JP20120286978

  • 发明设计人 富澤 稔;

    申请日2012-12-28

  • 分类号C12N5/071;C12N5/10;C12Q1/68;C12N15/09;

  • 国家 JP

  • 入库时间 2022-08-21 13:57:28

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