Methods for producing recombinant host cells, methods for producing pharmaceutical compositions, antibodies as mixtures, uses thereof, recombinant host cells, pharmaceutical compositions, methods for identifying antibodies as mixtures, and cultures of recombinant host cells

摘要

The invention provides methods for producing mixtures of antibodies from a single host cell clone. Thereto, a nucleic acid sequence encoding a common light chain, and nucleic acid sequences encoding two different heavy chains are expressed in a recombinant host cell. The antibodies in mixtures according to the invention comprise two different heavy chains capable of pairing to a common light chain, thereby forming functional antigen binding domains. Mixtures of antibodies, and use of these mixtures in a variety of field, are also provided by the invention.