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Method for changing the scanning field of a laser scanning microscope
Method for changing the scanning field of a laser scanning microscope
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机译:改变激光扫描显微镜的扫描场的方法
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摘要
Disclosed is a method for varying the size of the scanning field of a multifocal laser scanning microscope, said scanning field being scanned in X columns and Y lines, and n laser spots being arranged at a distance d from one another in the scanning field along the slow scanning axis in the sample plane, the distance between the scanned lines in the sample plane being a=d/K, where KεN, the size of the scanning field being varied by varying K. After scanning K lines, a vertical skip is made, e.g. a skip of (n−1)×K+1 lines in the scanning direction or (n+1)×K−1 lines against the scanning direction until at least Y lines have been scanned.
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机译:公开了一种用于改变多焦点激光扫描显微镜的扫描场的大小的方法,所述扫描场在X列和Y线中扫描,并且n个激光光斑在扫描场中沿着激光束彼此间隔距离d布置。样本平面中的慢扫描轴,样本平面中扫描线之间的距离为a = d / K,其中KεN,扫描场的大小通过改变K而变化。扫描K线后,进行垂直跳过,例如在扫描方向上跳过(n-1)×K + 1行或在扫描方向上跳过(n + 1)×K-1行,直到至少扫描了Y行。
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