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Method for examining a plurality of cultured cells for the presence of a periodic structure of at least one target component contained in the cultured cells

机译:检查多个培养细胞中是否含有培养细胞中至少一种靶成分的周期性结构的方法

摘要

The method for examining cultured cells for the presence of a periodic structure includes: a step of preparing a cultured cell; a step of fixing the cultured cell; and a step of coloring the fixed cultured cell using a first colorant. A step of stimulating the first colorant to emit light, a step of taking a two-dimensional image (2) of the fixed cultured cell, and a step of extracting from the image (2) of the fixed cultured cell Obtaining a correlation image (3) by cross-correlating the first filtered image and the second filtered image; and-maximum and minimum brightness values in the correlated image (3) Determining whether there is a periodic structure in a fixed cultured cell by determining whether there is a periodic structure of values.
机译:检查培养细胞中是否存在周期性结构的方法包括:制备培养细胞的步骤;以及制备培养细胞的步骤。固定培养细胞的步骤;以及使用第一着色剂使固定的培养细胞着色的步骤。刺激第一着色剂发光的步骤,拍摄固定培养细胞的二维图像(2)的步骤以及从固定培养细胞的图像(2)提取的步骤获得相关图像( 3)通过互相关第一滤波图像和第二滤波图像;以及相关图像中的最大和最小亮度值(3)通过确定值的周期性结构来确定固定培养细胞中是否存在周期性结构。

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