Pretreatment method for cell deployment device, cell deployment device, and cell deployment device pretreatment system

摘要

In order to improve the wettability of the cell deployment device in advance, when the prewet liquid is passed through the microchannel, the cell deployment device can be reliably improved in wettability by the prewet liquid. It is an object of the present invention to provide a treatment method, a device for cell deployment with improved wettability, and a pretreatment system for the device for cell deployment for improving wettability. The present invention is a cell expansion device in which a plurality of microchambers are formed, and a pretreatment method for a cell expansion device for removing bubbles remaining in the microchamber after a pre-wet liquid flow step, A microchamber chip forming step in which the volume per one microchamber and the total number of the microchambers are set in advance, a prewet liquid passing step in which a prewet liquid is passed through the microchannel, and a prewet After passing the liquid, a bubbled microchamber detecting step for detecting the number of microchambers in which bubbles are present, a bubble air volume calculating step for calculating the total amount A of bubbled air, From the sum of the total volume A and the volume B of air already dissolved in the pre-wet liquid, (A + B) / B is calculated. Then, by multiplying the calculated value by the normal pressure P to calculate the operating pressure X = P × (A + B) / B, and by applying the operating pressure X to the micro flow path, A bubble dissolving step of dissolving air existing as bubbles into the pre-wet liquid.