Provided is a method for separating a vesicle having a lipid bilayer membrane which is excellent in removal efficiency of contaminants and hardly destroys vesicles having a lipid bilayer membrane. Contacting a biological sample containing a vesicle having a lipid bilayer membrane with a solid phase carrier to which a ligand that recognizes a surface antigen present on the surface of the vesicle is bound to form a composite of the vesicle and the solid phase carrier A complex formation step of forming a body, and a washing step of washing said complex, wherein at least one of said complex formation step and said washing step is carried out in the presence of a nonionic surfactant A method for separating vesicles having a lipid bilayer membrane.
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