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Analyte Detection on a Solid Support by Nucleic Acid Amplification Coupled to an Immunoassay
Analyte Detection on a Solid Support by Nucleic Acid Amplification Coupled to an Immunoassay
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机译:核酸扩增结合免疫测定法对固相支持物进行分析物检测
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摘要
Embodiments of the invention provides a method for detection of at least one analyte derived from a sample, comprising the steps of: a) depositing the sample on a surface of a solid support; b) transferring at least a portion of the solid support to a receptacle suitable for performing a specific binding assay for one or more analytes of interest; c) optionally washing the portion; d) adding a single specific binding partner for each analyte of interest to the receptacle, the binding partner being labelled with an oligonucleotide sequence; e) mixing the portion with nucleic acid amplification reagents; f) amplifying the oligonucleotide sequence; and g) detecting amplified nucleic acid. The invention also provides a kit for use with the method for detection of at least one analyte derived from a sample.
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