Methods and Devices to Control Fluid Volumes, Reagent and Particle Concentration in Arrays of Microfluidic Drops

摘要

A microfluidic device includes a substrate comprising an inlet in fluid communication with one or more conduits and one or more parking loops in fluid communication with the one or more conduits. Each parking loop includes a bypass channel and a lower branch with a fluidic trap capable of retaining one or more drops of a sample solution, wherein the bypass channel has a smaller hydrodynamic resistance than the lower branch within the fluidic trap and a hydrodynamic resistance ratio (R_T/R_B) between the lower branch within the fluidic trap and the bypass channel is from 1.5 to 3.2. One or more outlets are in fluid communication with at least one of the bypass channels. A method for making the microfluidic device is also disclosed.