首页>
外国专利>
METHOD FOR SERUM-FREE CULTURE OF CHONDROCYTES AND SERUM-FREE CULTURE MEDIUM
METHOD FOR SERUM-FREE CULTURE OF CHONDROCYTES AND SERUM-FREE CULTURE MEDIUM
展开▼
机译:软骨细胞无血清培养的方法和无血清培养基
展开▼
页面导航
摘要
著录项
相似文献
摘要
To provide a method for serum-free culture of human cartilage cells and a serum-free culture medium. A method for serum-free culture of cartilage cells, said method comprising: an enzymatic treatment step for treating a human cartilage cell-containing tissue with a protease; an inhibitor-treatment step for, after the enzymatic treatment step, treating the tissue with an inhibitor for the aforesaid protease; and a culture step for, after the inhibitor-treatment step, culturing the tissue in a serum-free culture medium that contains kartogenin and/or SAG, ITS, FGF2 and hydrocortisone. A serum-free culture medium for culturing cartilage cells, said serum-free culture medium containing kartogenin and/or SAG, ITS, FGF2 and hydrocortisone.
展开▼