SIMULTANEOUS PLURAL COLOR BROADBAND COHERENT ANTI-STOKES RAMAN SCATTERING MICROSCOPE AND IMAGING

摘要

A plural color broadband coherent anti-Stokes Raman scattering (CARS) microscope includes: a first light source to produce a first light including a narrowband radiation; a second light source to produce a second light including a broadband radiation; a third light source to: receive the first light from the first light source; receive the second light from the second light source; and produce a third light comprising the narrowband radiation and the broadband radiation by combining the first light and the second light such that the first light and second light are spatially overlapped and temporally overlapped; and a primary objective to: receive the third light from the third light source; communicate the third light to a sample; and subject the sample to simultaneous interpulse CARS stimulation and intrapulse CARS stimulation by irradiation with the narrowband radiation and the broadband radiation in the third light.