METHOD FOR DETECTING GUANINE-ABASIC SITE IN DNA

摘要

The present invention provides a method for detecting the presence or absence of a guanine-abasic site, the method being a process for detecting guanine opposite at least one abasic sites generated in a double-stranded DNA, comprising:(1) step 1 of site-selectively cleaving at least one abasic sites in a double-stranded DNA using an enzyme;(2) step 2 of modifying the amino group at position 2 of guanine opposite the abasic sites using a modifier; and(3) step 3 of performing polymerase chain reaction on the modified double-stranded DNA obtained by conducting step 1 and step 2, which serves as a template, to search for the presence or absence of an amplification product, the sequence of steps 1 and 2 being not limited to the order presented.