MEDIUM RETAINING CHAMBER AND CHAMBER SYSTEM FOR GROWTH AND MICROSCOPIC OBSERVATIONS OF CULTURED CELLS

摘要

A chamber for a cell culture and a chamber holder system are disclosed. A representative chamber embodiment includes a first layer; and a second layer coupled to the first layer, the second layer further comprising a well having at least one side wall, the well extending through the second layer, wherein a predetermined portion of the first layer is substantially optically transmissive and is exposed in and forms a lower side of the well. The well may have a laminar flow shape, and may also include a plurality of recesses to accommodate the tips of inflow and outflow devices, such as for superfusion applications. The second layer may be comprised of a hydrophobic material or comprised of a material having a lower density than the density of culture medium to provide a buoyant chamber for sandwich cell cultures, along with submersible chambers.