Protease assay method using site-specific fluorescence dye labeled protein as substrate

摘要

A method for a fluorescence based assay that includes providing a full length protein substrate, attaching a detection component to a specific site of the full length protein substrate to provide a site-specifically labeled full length protein substrate, interacting the site-specifically labeled full length protein substrate with an analyte of interest; and performing an assay for drug discovery using the site-specifically labeled full length protein substrate and the analyte of interest.