DNA sequencing by synthesis using Raman and infrared spectroscopy detection

摘要

This invention provides nucleoside triphosphate analogs having the structure:; wherein B is a base and is adenine, guanine, cytosine, uracil or thymine, wherein R″ is an OH or an H, and wherein R′ is azidomethyl, a hydrocarbyl, or a substituted hydrocarbyl, and which has a Raman spectroscopy peak with wavenumber from 2000 cm⁻¹ to 2300 cm⁻¹ or a Fourier transform-infrared spectroscopy spectroscopy peak with wavenumber from 2000 cm⁻¹ to 2300 cm⁻¹, and also to methods of DNA sequencing and SNP detection.