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Cross-linking compositions and related methods of isotope tagging of interacting proteins and analysis of protein interactions
Cross-linking compositions and related methods of isotope tagging of interacting proteins and analysis of protein interactions
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机译:相互作用蛋白的同位素交联组成及相关方法及蛋白相互作用分析
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摘要
An isotope labeled asymmetric cross-linker is provided for the detection of cross-linked peptides. A cross-linking and mass spectrometry strategy, referred to as isotope tagging of interacting proteins (iTIP), improves the specificity of detecting cross-linked peptides and accurate identification of the interacting peptide sequences via the incorporation of isotopic signatures that are readily observed in the MS/MS spectrum. Isotope tagged peptides can be identified using mass spectrometry based on doublet peaks in a spectrum. Spectra can be subjected to database search strategies available for the sequencing of linear or non-cross-linked peptides.
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机译:提供了同位素标记的不对称交联剂,用于检测交联的肽。交联和质谱分析策略,称为相互作用蛋白的同位素标记(iTIP),通过掺入同位素标记(在反应中容易观察到),提高了检测交联肽和特异性鉴定相互作用肽序列的特异性。 MS / MS谱图。可以使用质谱基于光谱中的双峰来鉴定同位素标记的肽。可以对Spectra进行数据库搜索策略,以对线性或非交联肽进行测序。
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