A method for making specific amendments in the whole Plant by Transient gene expression

摘要

This application discloses a method to make specific changes in the whole Plant site by Transient expression of genes.The Method provided for the conduct of site specific Modification of a Target Fragment of a Target Gene in a whole Plant comprises the following steps: The Transient expression of a specific sequence of Nuclease in that plantWhere It is used as the subject for the whole Plant Transient expression; Sequence specific Nuclease that is directed to the target fragment and Split; this is achieved through site specific Modification of DNA Self repair of the plant.At present, it omits the tissue culture by Transient expression of the Nuclease specific Sequence; mutation is obtained at the level of the whole Plant. The method is independent of the genotype and the receiver, and therefore can be applied to a variety of Dive Rsas species; The mutants T1 can be obtained directly.And The Mutation can be stably inherited; more important, the obtained Mutant plant is free of exogenous genes and therefore has Greater Biosecurity.