Method for making site directed changes in the Genome of a Plant using non Heritable materials

摘要

The application discloses a method for driving modifications targeted site in the plant Genome, using materials not Heritable.The Method provided in this application specifically includes the following Stages: the introduction of a material not Heritable in a cell or in a tissue, or a part of the plant of interest, where such material is a non Heritable specific Nuclease for that fragment objet Ivo or mRNA expresses the Nuclease.By which the objective fragment is Dissociated by the Nuclease, and accomplished the modification site directed at the target fragment through DNA Repair in Plant.Through the introduction of a material not inheritable Nuclease can be Sequence specific, site directed mutation in the Gene of a Plant, and will not be integrated Nucleic Acid Fragments or foreign genes in plant is obtained. Therefore, this application may lead to a study of the role of the Genome more precise, and more comprehensive cultivation.