The production process of mananase of microbial origin, using industrial waste by-products /

摘要

The production process of mananase of microbial origin, using industrial waste by-products / the mananases are enzymes that cleave the chains of 10 mananas, present in cell wall fraction hemicelulu00f3sica plant, having applications in various industries: Food and beverages. The pulp and paper, textile, soap and detergents, animal feed, among others.The present invention is the use of shells of soybean to obtain enzyme mananase through solid state fermentation in 15 (FES) and semi - solid (FESS), using a non genetically modified micro organism.For both, the pre inoculum from non genetically modified micro organism Aspergillus niger LPB 28 is obtained after cultivation in 50 ml of agar locust bean gum (144 hours / 28 u00b0 C), and inoculated (1 X1 07 spores / g of soybean hulls drought) in a sterile container 20 with shells of soybeans plus solutions. Will be nourishing composed of urea (0.7 g / L), manganese sulphate (LO g / L) and sucrose (20 g / L),Using 70% of initial humidity to the process of FES (96 hours / 28 u00b0 C), and 15% of Soybean Hulls in proportion to m / V solution nutritivapara process of FESS (144 hours / 28 u00b0 C / under constant agitation). The top 25 mananase yields are approximately 200 u g - 1 for FES and 3600 U L - 1 for FESS, reaching nearly 6200 u g - 1 after lyophilization.