DNA construction, eukaryotic host cell, eukaryotic cell line and process of preparing a target protein

摘要

abstract there is provided inter alia a dna construct which comprises a trnapyl coding sequence and a rna polymerase iii promoter sequence which is capable of acting to functionally functional trnapyl sufficiently to support nonsense suppression in a eukaryotic expression system. abstract of the patent summary: "nucleic acid molecules". inter alia, a DNA construct comprising a trnapyl coding sequence and an rna polymerase III promoter sequence that is capable of acting to express functional trnapyl sufficiently to support meaningless suppression in a eukaryotic expression system is provided.