chimeric protein, oligomeric protein complex, polynucleotide, vector, transgenic cell, pharmaceutical composition, chimeric protein production method, method for producing an antibody, bacterial load reduction method, resistance induction method, vaccine, chimeric protein use, polyclonal antibody, method of prevention and use of antibody

摘要

Brucella lumazine synthase chimeras and beta subunit chimeras of ab5 toxins The present invention relates to chimeric polypeptides useful as immunogens for inducing immunoprotective responses and neutralizing antibodies against shiga toxin (stx) in mammals. More specifically, the present invention relates to chimeric polypeptides comprising a type 2 shiga toxin hemopentamer b-subunit monomer fused to the n-terminal of a brucella lumazine synthase monomer and oligomeric protein complexes formed from said chimeric polypeptides. The invention further relates to polynucleotides and vectors encoding said chimeric polypeptides, transgenic cells comprising said polynucleotides and vectors, and pharmaceutical compositions as a vaccine comprising said chimeric polypeptides and chimeric polynucleotides. Also within the scope of the invention are antibodies that bind to the chimeric polypeptides of the invention, a method for obtaining antibodies that specifically bind to type 2 shiga toxin subunit B and methods of reducing the bacterial burden of escherichi coli entero -hemorrhagic in mammals, which can be used to prevent, among other things, hemolytic uremic syndrome (hus).