The development of biotechnology of based formulation of Croton alkaloids cajucara Benth for treatment of pain and inflammation.

摘要

Croton cajucara Benth is a plant of great representation in northern Brazil, and has gained prominence in the scientific scenario for its beneficial effects on health, which led to its inclusion in the list of medicinal plants of sus (Unified Health System).The objective of this patent was the preparation of a biomass consists of a polar fraction (alcalou00eddica, F3) isolated from ethanol water extract of bark of stem of croton cajucara Benth, conveyed to a system microemulsionado type oil in water (O / a) with characteristic automicromuls Ificante (SMEDDS), for the production of MEF3 byproduct.The system carreador was obtained with a mixture of surfactant in the class of sorbitanos (tweens) and a vegetable oil (rich in organic acids Prefecture and poly - insatuardos). The assessment of analgesic and anti-inflammatory activity of the bioformulado MEF3 was carried out via experimental protocols in vivo (n = 6, voiceover).Pre treatment with MEF3 in doses of 1 mg / kg, 3 mg / kg and 5 mg / kg, reduced abdominal contortions in 67.4%, 70.2% and 72.5% respectively compared to the control group.The rate of inhibition of leukocyte migration related to inflammation induced by carrageenan, showed that oral administration of bioformulado MEF3, reduced neutrophil migration into the peritoneal cavity at 16.5%, 25.9% and 39.9% in doses of 1 mg / kg, 3 mg / kg and 5 mg / kg resp Ivamente.Based on the experimental models, biomass MEF3 is indicated for the treatment of chronic inflammatory diseases such as arthritis, fibromyalgia, arthritis, colitis ulcerativas, among others.