stromal cell derived conditioned medium, method of obtaining said conditioned medium compositions, formulations and applications thereof

摘要

abstract "stromal cell derived conditioned medium, method of obtaining said conditioned medium compositions, formulations and applications thereof" bioactive factor enriched conditioned (cm) medium, its composition and the method of production of cm are disclosed. The method of obtaining the desired amount of bioactive factors in conditioned medium comprises pooling bone marrow-derived mesenchymal stem / stromal cells, cultivating the clustered cells and subjecting the cell culture to a specified confluence cell feeding schedule and collecting the conditioned medium. potent rich in bioactive factors in specific passage / time period. The method also aims to maximize the probability of generating a conditioned medium with reduced biological variability and comprising a large number of bioactive factors that have specific biological property / function. The disclosure also relates to a composition and formulation of the conditioned medium and its use in therapeutic and cosmetic areas. 1/1