The use of a protease family of peptidases S2A and / or fmu00edlia of peptidases S1E, method for producing the polypeptide, the additive of animal feed, animal feed composition, and detergent composition.

摘要

"The protease family of peptidases S2A and / or family of peptidases S1E, sequence of nucleic acid isolation, construction of expression vector of recombinant nucleic acid.Recombinant host cell, method for producing a polypeptide transgenic plant or plant part, animal transgenic non-human or products or elements of the same.Use of at least one polypeptide, additive of animal feed, animal feed composition, and detergent composition.The invention relates to a new class of serine proteases family of peptidases S2A or S1E which are stable in the presence of copper (Cu2 +) and / or are inhibited by copper only in degree reduced.Structural features of potential relevance for this purpose are also disclosed.This class of proteases include proteases derived from brachysporiella gayana, Nocardiopsis dassonvillei subspecies dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba.But also excludes known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262.The invention relates to DNA encoding these proteases, the expression thereof in a host cell.Including the invention relates to a new class of serine proteases family of peptidases S2A or S1E which are stable in the presence of copper (Cu2 +) and / or are inhibited by copper only in degree REduzido. Structural features of potential relevance for this purpose are also disclosed.This class of proteases include proteases derived from brachysporiella gayana, Nocardiopsis dassonvillei subspecies dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba.But also excludes known proteases derived from metarhizilu00edm anisopliae and Nocardiopsis sp. NRRL 18262.The invention relates to DNA encoding these proteases, the expression thereof in a host cell, including cells of plants and animals, as well as to its use.For example, in animal feed and in detergents.Particularly, the invention relates to animal feed and animal feed additives, such as pre mix, incorporating these proteases together with 1 - 500 ppm Cu (concentration in feed).