Nanoparticles for magnetic and fluorescence labelling of cells, the preparation and production process and their use

摘要

ufeffzpu016fsob marking of cells and imaging kobaltnatozineu010dnatu00fdch feritovu00fdch multifunctional aqueous suspensions of nanoparticles. the hybrid nanoparticles spinelovu00e9, ferrite phase of general formula co.sub.1-x.n.zn.sub.x.n.fe.sub.2.n.o.sub.4 + y.n.where x is 0.2 to 0.8, with a continuous layer of hydrated silicon dioxide with navu00e1zanou fluorescent mark, with the advantages of fluoresceinisothiokyanu00e1tu (fitc), which, on the one hand, ensures the stability of the aqueous suspensions and innocuity of biokompatibilnu00edm surface and its on the one hand, allows the imaging of cells, fluorescent microscope.compared with the existing materials exhibit significantly increased transversu00e1lnu00ed relaxivitu and thus higher contrast when displaying magnetic resonance imaging. they are therefore useful in biology and medicine for the marking of transplanted cells, especially mezenchymovu00fdch stem cells, because the marking does not affect their viabilitu or differentiation potential.another advantage is that the presence of nanoparticles is not in cell dna damage, lipid and protein. they are therefore suitable for in vivo monitoring of transplanted cells by magnetic resonance imaging and optical methods.