Purification process of betulin and lupeol and betulin and lupeol prepared thereby

摘要

ufeffpu0159edklu00e1danu00e9 solution relates to the purification of betulin and lupeolu mixture triterpenic esters birch bark extract, which contains the following steps: (a) the extract mixture of triterpenic esters birch bark is dissolved in acetone, with benefit at boiling point; (b) the solution to heat filtered, fi ltru00e1t is diluted with distilled water, the resulting suspension is filtered.washed with distilled water and dried; (c) a clot formed in step (b) is dissolved in ethanol to the boiling point, add the hot solution to a solution of cacl.sub.2.n.v ethanol and thus combined the clear solution is slowly added hot naoh solution in ethanol, the gradual formation of insoluble ca (oh) .sub.2.n.that filters out his, and the filtrate left to crystallize, the crystalline precipitate is filtered, washed with ethanol and water and dried; (d) the crystalline precipitate formed in step (c) is dissolved in chloroform, with benefits for boiling, and the solution precipitates out with light petroleum and / or petrol and / or a low hydrocarbon selected from the group containing, (c5 through c8) hydrocarbons, with advantage of pentane.hexane, cyclohexane, heptane, and the resulting precipitate of betulin is filtered, washed with light petroleum and / or petrol and / or a low hydrocarbon selected from the group containing, (c5 through c8) hydrocarbons, with advantage of pentane, hexane, cyclohexane, heptane, and dried in a process vau0159e herfiltration and drying is repeated at least once with advantage; obtained filtrate containing lupeol evaporate; (e) a clot or filtrate from the step (d) is dissolved in a minimum amount of chloroform at ambient temperature, the resulting solution is selectively filtered through silica gel at chloroform and colourless filtrate evaporated dry.the solution also involves the betulin and lupeolu pu0159ipravitelnu00fdch in this way.