method of determination of u0441u0438u0430u043bu043eu0432u044bu0445 acids in biological fluids

摘要

the invention belongs to the field of medicine, namely clinical laboratory diagnosis and can be used to quantify the content of si u0430u043bu043eu0432u044bu0445 acids in biological fluids.the essence of the invention is a method of determining the u0441u0438u0430u043bu043eu0432u044bu0445 acids in biological fluids is that, in the original mixture, consisting of a saliva sample for preparation of the mixture are in a 1 ml of solution u0442u0440u0438u0445u043bu043eu0440u0443u043au0441u0443u0441u043du043eu0439 acid with concentration of 100 g / l, 2 ml of distilled water and 0.3 ml of saliva and them carefully mix,then a reference mixture placed in boiling water bath, and retain it for 5 min, followed by cooling to room temperature, after which the u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u0443u044eu0442 within 10 min at a speed of rotation of the centrifuge, 2500 rpm and then select from 2 ml u043du0430u0434u043eu0441u0430u0434u043eu0447u043du043eu0439 liquid, add zero4 ml u0443u043au0441u0443u0441u043du043e - u0441u0435u0440u043du043eu043au0438u0441u043bu043eu0439 mixture and re placed in the boiling water bath and keep it for 15 min, then cooled to room temperature, u0434u043eu0431u0430u0432u043bu044f s 2 ml of distilled water and measure the optical density of the solution against the blank samples, which is in the form of a solution of sulphuric acid in the u043bu0435u0434u044fu043du043e in acetic acidgreen u0441u0432u0435u0442u043eu0444u0438u043bu044cu0442u0440u043eu043c (500 to 560 nm) in the ditch layer with thickness of 10 mm, and determine the concentration of u0441u0438u0430u043bu043eu0432u044bu0445 acids are on u0433u0440u0430u0434u0443u0438u0440u043eu0432u043eu0447u043du043eu043cu0443 timetable system t has no less than five points, using a standard solution of n - u0430u0446u0435u0442u0438u043bu043du0435u0439u0440u0430u043cu0438u043du043eu0432u043eu0439 acid at concentrations from 0.050 to 1.00 mmol / l.the technical result, which is implemented by using of the proposed method is to simplify the method, improving the efficiency and reducing the u043cu0430u0442u0435u0440u0438u0430 u043bu043eu0435u043cu043au043eu0441u0442u0438.