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CLONATION, EXPRESSION AND USE OF ACID LYSOPHOSPHOLIPASES
CLONATION, EXPRESSION AND USE OF ACID LYSOPHOSPHOLIPASES
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机译:酸性溶血磷脂酶的克隆,表达及用途
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摘要
DNA sequence (I) coding for an Aspergillus fumigatus lysophospholipase (II) is new. DNA sequence (I) having SEQ ID NO:1 (defined sequence of 2507 nucleotides given in the specification) and coding for a lysophospholipase (II) with SEQ ID NO:2 (defined sequence of 611 amino acids given in the specification) is new. Independent claims are also included for: (1) expression construct comprising (I) linked to regulatory sequences for expression in a host cell; (2) recombinant host cell transformed with the expression construct; (3) plasmid B6Sma35 (DSM 18370); (4) phospholipase encoded by the coding portion (especially nucleotides 580-2367) of (I); (5) producing (II) by culturing a host cell as above.
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机译:编码烟曲霉溶血磷脂酶(II)的DNA序列(I)是新的。具有SEQ ID NO:1(说明书中指定的2507个核苷酸的定义序列)并编码具有SEQ ID NO:2(说明书中指定的611个氨基酸的溶血磷脂酶)(II)的DNA序列(I)是新的。还包括以下方面的独立权利要求:(1)表达构建体,其包含(I)与调节序列连接以在宿主细胞中表达; (2)用表达构建体转化的重组宿主细胞; (3)质粒B6Sma35(DSM 18370); (4)由(I)的编码部分(特别是核苷酸580-2367)编码的磷脂酶; (5)通过如上所述培养宿主细胞来产生(II)。
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