A METHOD OF PREDICTING RELATIVE FED BATCH PRODUCTION TITER OF A PANEL OF CLONALLY DERIVED PRODUCER CELLS

摘要

A rapid high throughput method of predicting relative fed batch performance of a panel of clonal cells derived from a single host cell line. A computer implemented method of predicting relative fed batch production titer of a panel of clonal producer cells derived from a single parental host cell population comprises the steps of assaying a level of growth of each clone in the presence and absence of at least three individual chemical cell stressors comparing the level of growth of each clone in the presence and absence of the at least three chemical cell stressors to provide a normalised production titer response for each clone in each stressed microenvironment and inputting a clone specific production titer response profile comprising the normalised production titer response for each clone in each stressed microenvironments into a computational model. The computational model is generated from production titer response profiles obtained from a calibration set of clones with known fed batch production titer and is configured to output the predicted relative fed batch production titer of the panel of clonal cells.