Method for determining the sex of Actinidia arguta plants cultivated in vitro

摘要

The invention relates to biotechnology, particularly to a method for determining the sex of Actinidia arguta plants cultivated in vitro.The method, according to the invention, consists in determining the electrophoretic peroxidase spectrum in the plant leaves, obtained by inoculation of lateral buds of minicuttings and cultivation of the obtained shoots with rhizogenesis induction on a nutrient medium, by maceration of plant leaves in 0.05 M Tris-HCl buffer solution, subsequent mixing and separation of the extract by centrifugation, disc electrophoresis of the extract in tubes with polyacrylamide gel to obtain the electrophoretic peroxidase spectrum and staining of bands, at the same time if the obtained spectrum contains 8 peroxidases, the plant is of feminine gender, and if it contains 4 peroxidases - of masculine gender.