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RECOMBINANT STRAIN OF SACCHAROMYCES CEREVISIAE OVERPRODUCING GLYCEROL.
RECOMBINANT STRAIN OF SACCHAROMYCES CEREVISIAE OVERPRODUCING GLYCEROL.
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机译:酿酒酵母过表达甘油的重组菌株。
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摘要
A truncated version of Saccharomyces cerevisiae IVL2 gene encoding a cytosolic form of acetolactate synthase was cloned into an expression cassette under control of a strong constitutive alcohol dehydrogenase (ADH1) promoter. The plasmid was introduced into the S. cerevisiae strain and the recombinant strain was tested for ability to overproduce glycerol under anaerobic conditions. It was shown that the recombinant strain was characterized by increased glycerol production and decreased ethanol production under anaerobic conditions.
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