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Non-transgenic haploid inducer lines in cucurbits.

机译:葫芦丝中的非转基因单倍体诱导物系。

摘要

The present invention relates to a mutant plant of the Cucurbitaceae family comprising a modified CENH3 gene, which mutant plant, when crossed to a wild-type plant having 2n chromosomes produces progeny, at least 0.1% of which have n chromosomes. Preferably, the modified CENH3 gene comprises at least one mutation compared to an otherwise identical naturally occurring CENH3 gene, which at least one mutation gives rise to at least one nonconservative amino acid change in the Histone Fold Domain of the encoded modified CENH3 protein or to the occurrence of a premature stop codon in the encoded modified CENH3 protein. The invention further relates to a method for the production of haploid or doubled haploid plants and to the plants thus obtained.
机译:本发明涉及葫芦科的突变植物,其包含修饰的CENH3基因,该突变植物与具有2n个染色体的野生型植物杂交时产生后代,其中至少0.1%具有n个染色体。优选地,与在其他方面相同的天然CENH3基因相比,修饰的CENH3基因包含至少一个突变,该至少一个突变在编码的修饰的CENH3蛋白的组蛋白折叠结构域中或在编码的修饰的CENH3基因的组蛋白折叠结构域中引起至少一个非保守氨基酸变化。编码的修饰CENH3蛋白中过早终止密码子的出现。本发明进一步涉及生产单倍体或双倍单倍体植物的方法以及由此获得的植物。

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