Genetic variability of anther donor versus spontaneous doubled haploid descendents and colchicine induced doubled haploid sweet pepper (Capsicum annuum L.) lines

摘要

Haploid (n) and doubled haploid (DH) plants were developed in anther culture of sweet pepperud(Capsicum annuum L.). Regenerants were analyzed by flow cytometry for haploid (n = 12)udand spontaneous doubled haploid (2n = 24) genomes. Haploid plants were forwarded toudcolchicine-treatment for induced doubled haploid (2n·) plant production. Molecularudpolymorphism of anther donor plants (2n), the haploid regenerants (n), the spontaneous (2n) andudinduced (2n·)-DH plants were analysed by RAPD-, SSR- and ISSR-PCR. The analysis ofudanther-donor plants compared to DH-descendents showed an unexpectedly wide range ofudmolecular polymorphism. Our results suggest that genetic changes occurring during meioticudrecombination is higher than those of occurring during colchicine-induced genomic duplication.