Method for preparation of inoculum for cellulolytic bacteria for the production of biopreparations to improve soil properties

摘要

how to prepare inoculum bacteria celulolitycznych to manufacture biopreparatu00f3w improve soil properties, including soil bacteria isolation of bacillus, characterised by the fact that with the substrate for the production of mushrooms or mushroom extracts s after the production of the substrate one sample is mixed with a sterile saline solution containing nacl.the proportion of mass samples to the salt solution from 1: 5 to 1: 20 and performs a series of dilutions of the suspension of concentrations from 1 to 10 - 10 - 15. then is withdrawn from the suspension of any dilution of a volume of at least 0,1 ml and transfer vessel for breeding microorganisms in liquid medium containing 1%. polypeptides casein, 1%.carboxymethyl cellulose, 0,2%. k2hpo4, 1,5%. agar, 0,03%. mgso4u20227h2o and 0.25%. (nh4) 2so4, 0,2%. gelatine and demineralized water to 100% and is multiplication of bacteria at a temperature not lower than between 15 oc for at least two hours.this process is repeated until a single colony of bacteria bacillus undergo colonies analysis enzyme and subsequently isolating cell colonies showing the highest activity celuloitycznu0105, judging them on skosach agarowych with solid medium with broth and kept at a temperature of at least - 20u00b0c.